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Herpes simplex virus belongs to the α subfamily of human herpesviridae. According to the difference of G protein, it can be divided into two serotypes: Herpes Simplex Virus Type 1(HSV1) and Herpes Simplex Virus Type 2 (HSV2). The nucleic acid sequences of the two types have high homology, but the infection way and clinical manifestations are different. The recurrence rate of genital herpes caused by HSV2 infection is much higher than that caused by HSV1, so it has important clinical significance for the diagnosis and detection of HSV.

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According to the characteristics of nucleic acid sequence of herpes simplex virus type 1 and herpes simplex virus type 2, two sets of specific primers and fluorescent probes were designed by selecting a conserved region within type andspecific region between type respectively. The primers and probes in each group could bind to the corresponding target sequence specifically withoutcross-reaction. At the same time of PCR amplification reaction, the fluorescent signal generated by the excitation light of the fluorescent probe can be detected bythe automatic fluorescent PCR detector to realize real-time online monitoring of the PCR reaction. In the extraction and detection process, human RnaseP gene (IC) is introduced as a non-competitive internal reference to control the whole extraction and detection process, In addition, UDG enzyme anti-contaminationmeasures are added in this kit to fully decompose possible product contamination and avoid false positive results.


• Strong specificity

No cross-reaction between HSV1 and HSV2. And no-cross reaction with  similar viruses such as group B streptococcus, HPV16, HPV18, human  cytomegalovirus, Epstein-Barr virus adenovirus and so on.

• Strong anti-interference ability

Blood (10%), mucin (0.2mg/mL), cervical mucus (15%), vaginal lubricant  (3%), gynecological cleanser (5%), azithromycin (0.4mg/L), levofloxacin (5μg/mL) have no interference on the test results of the kit.

• Simple operation

The two viruses can be detected and identified time in one reaction, and the  detection can be completed within 32 min.


HSV1 and HSV2 test




-20 ± 5 ℃ avoid light