This kit was designed to detect the relatively conserved region of eight pathogens genome respectively by specific primers and fluorescent probes and performs rapid quantitative detection of the pathogens DNA by qPCR after the nucleic acid extraction process. In the extraction and detection process, human RnaseP gene was introduced as a noncompetitive internal control to monitor the whole extraction and detection process. In addition, UDG enzyme anti-contamination system was added in the kit to fully decompose possible product contamination and avoid false positive results. The kit mixes all the components in advance, and the experiment can be carried out without additional preparation actions, which is simple and convenient to use.
• Specimens: Male urethral swabs and female cervical swabs.
• Simplicity: Only need one nucleic acid extraction, simultaneously detect eight STD pathogens.
• Specificity: No cross reactivity has been observed by testing the clinical positive specimens such as Group B streptococcus, human papilloma virus, Salmonella, Pseudomonas aeruginosa, Escherichia coli, Treponema pallidum, Human cytomegalo virus, Epstein-Barr virus, Human herpes virus type 6, Candida albicans, Pneumonia mycoplasma, Bordetella pertussis, Staphylococcus aureus, Candida glabrata and Adenovirus.
• Real-time monitoring: Human RnaseP gene was introduced as a non-competitive internal control to monitor the whole extraction and detection process.
• Anti-contamination system: UDG enzyme was added in the kit to fully decompose possible product contamination and avoid false positive results.
• Easy to use: Premixed PCR Mix, the experiment can be carried out without additional preparation actions, which is simple and convenient to use.
STD Pathogen Multiplex Detection Kit (Fluorescent PCR) is used for the qualitative detection of eight pathogens in sexually transmitted disease, including Chlamydia trachomatis (CT), Neisseria gonorrhoeae (NG), Mycoplasma genitalium (MG), Herpes simplex virus 1 (HSV-1), Herpes simplex virus 2, Gardnerella vaginalis (GV) , Ureaplasma urealy, Ticummonas vaginalis (TV) in male urethral swab and female cervical swab.
1. The kit can be transported in foam box sealed with ice bags or dry ice below 8℃.
2. The kit should be stored at -25℃~-15℃ away from light and avoid repeated freeze-thaw more than 5 times. The kit can be stored for 7 days at 2 ~8℃ after opening.
3. The kit can be stored for up to 12 months if all components are kept in the manner above. (Please use the kit in the period of validity).