This product selects the VP6 gene (FAM) regions of Group A Rotavirus, Hexongene (HEX) regions of Enteric Adenovirus and ORF1 gene (ROX) regions of Norovirus, and designs four sets of primers and fluorescent probes that cover foursites of the genes. The four sets of primers and probes can specifically bind to thetarget sequences. When the RT-PCR amplification reaction is performed, thefluorescent signal (s) can be detected by a full-automatic fluorescent PCR detectorto realize real-time online monitoring of the RT-PCR reaction. In order to controlthe entire extraction and detection process, human gene (Cy5) was act as anon-competitive internal control during the extraction and detection process.
� Rapid
It can accurately detected norovirus, group A rotavirus and enteric adenovirus in 35 minutes together with Bioer FQD qPCR instruments.
� High sensitivity
500 copies/mL.
� Strong applicability
Applicable to human stool specimens.
� Strong specificity
No cross reaction with adenovirus 3, coxsackie A2, enterovirus 71, coxsackie A10, adenovirus 7, Vibrio parahaemolyticus, Escherichia coli 30, Bacillus cereus, Escherichia coli and Staphylococcus aureus; The possible triglycerides, hemoglobin, white blood cells and mucin in the sample have no effect on the detection of the sample.
� High accuracy
Results from reference materials: positive coincidence rate 100%, negative coincidence rate 100%.
� Simple operation
Only 35min complete the RT-PCR in one step, the amplification and detection progress in the closed tube to prevent aerosol pollution.
1. The kit should be stored at -25°C - -15°C away from light and avoid repeated freeze-thaw.
2. The kit can be stored for 3 days at 2-8 °C after opening.
3. The kit can be stored for up to 12 months if all components are kept in themanner above. Do not use after the stated expiry date.
4. The kit can be transported in foam box sealed with ice bags or dry ice under 8 °C.
