According to the characteristics of nucleic acid sequence of herpes simplex virus type 1 and herpes simplex virus type 2, two sets of specific primers and fluorescent probes were designed by selecting a conserved region within type andspecific region between type respectively. The primers and probes in each group could bind to the corresponding target sequence specifically withoutcross-reaction. At the same time of PCR amplification reaction, the fluorescent signal generated by the excitation light of the fluorescent probe can be detected bythe automatic fluorescent PCR detector to realize real-time online monitoring of the PCR reaction. In the extraction and detection process, human RnaseP gene (IC) is introduced as a non-competitive internal reference to control the whole extraction and detection process, In addition, UDG enzyme anti-contaminationmeasures are added in this kit to fully decompose possible product contamination and avoid false positive results.
• Strong specificity
No cross-reaction between HSV1 and HSV2. And no-cross reaction with similar viruses such as group B streptococcus, HPV16, HPV18, human cytomegalovirus, Epstein-Barr virus adenovirus and so on.
• Strong anti-interference ability
Blood (10%), mucin (0.2mg/mL), cervical mucus (15%), vaginal lubricant (3%), gynecological cleanser (5%), azithromycin (0.4mg/L), levofloxacin (5μg/mL) have no interference on the test results of the kit.
• Simple operation
The two viruses can be detected and identified time in one reaction, and the detection can be completed within 32 min.
HSV1 and HSV2 test
