This product selects the pl (FAM) region of MP and designs a pair of primers and a fluorescent probe. The primer and probe can specifically bind to the target sequence. When the PCR amplification reaction is performed, the fluorescent signal(s) can be detected by a full-automatic fluorescent PCR detector to realize real-time online monitoring of the PCR reaction.
The reaction system of this kit contains the dUTP-UDG enzyme anti-pollution system to avoid false positive results; at the same time, in order to control the entire extraction and detection process, human gene was act as a non-competitive internal control during the extraction and detection process.
• Strong applicability: Suitable for human pharyngeal swabs.
• High sensitivity: Three different batches of reagents were used to test and the sensitivity can reach to 200 copies/mL.
• Good specificity: No cross reactivity has been observed by testing the clinical positive specimens such as Ureaplasma Urealyticum, Influenza A (HIN1), Influenza B (Victoria), Respiratory Syncytial Virus A/B, Parainfluenza Type 3，etc.
• Excellent anti-interference ability: Endogenous inhibitors (such as blood, mucin, and nasal secretions) and exogenous inhibitors (such as common drugs for treating colds or other similar symptoms) showed no influence on the detection.
• High Precision: Positive controls and low positive controls reference were tested by 3 lots of kits with 10 replicates by 2 operators for 20 days. The results showed that the variation coefficient (CV) of within-day, between-day, within-batch and between-batch were less than 5%.
• Simple operation: The PCR test can be finished within 32 min.
Mycoplasma pneumoniae (MP) test
The kit can be stored for 5 days at 2-8℃ after opening.
