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This product selects the ORFlab and N gene ((FAM)) regions of SARS-CoV-2 the specific regions of Influenza A Virus (HEX) and Influenza B Virus (ROX) and designs four sets of primers and fluorescent probes that cover four sites of thegenes. The four sets of primers and probes can specifically bind to the target sequences. When the RT-PCR amplification reaction is performed, the fluorescent signal (s) can be detected by a full-automatic fluorescent PCR detector to realize real-time online monitoring of the RT-PCR reaction. In order to control the entire extraction and detection process, human gene (Cy5) was act as a non-competitive internal control during the extraction and detection process.

Product Details FAQ Specification Instructions

The main symptoms of the novel coronavirus pneumonia are fever and dry cough. Some people will have other symptoms such as fatigue, muscle aches, shortness of breath, diarrhea, etc., but the symptoms such as runny nose and sputum are rare. Influenza A virus is the most common influenza virus. It usually presents with influenza-like symptoms, including fever, sore throat, runny nose, nasal congestion, cough, phlegm, headache, general soreness, fatigue, vomiting and diarrhea in some cases. Influenza B virus often causes localized epidemics, patients will have general malaise, high fever, myalgia and other symptoms, serious patients will have some complications, such as encephalitis, pneumonia and so on.

The clinical symptoms and complications caused by novel coronavirus SARS-CoV-2 and influenza A and influenza B are similar, and they are infectious and harmful. The novel coronavirus SARS-CoV-2 and influenza A and B virus nucleic acid detection kit (fluorescent PCR) can simultaneously detect the infection of SARS-CoV-2, influenza A and influenza B viruses, especially in the stage of viral infection to effectively distinguish virus types, which is conducive to the prevention and treatment of novel coronavirus and influenza A and B viruses. It is of guiding significance for the diagnosis and treatment of novel coronavirus and influenza A/B virus. At the same time, it also has the characteristics of economy and efficiency, and maximizes the allocation of medical resources.


Advantage: This kit only takes 35 min for a single reaction, which can qualitatively detect the presence of novel coronavirus SARS-CoV-2 and influenza A and B viruses in clinical samples and can be used for rapid diagnosis of humans with related suspected symptoms.
Strong applicability: Suitable for human nose swab, throat swab.

Strong specificity: With diplococcus meningococcus, Haemophilus influenzae, Staphylococcus aureus, Streptococcus pneumoniae, rubella virus, mumps virus, respiratory adenovirus (type 3), respiratory adenovirus (type 7), respiratory syncytial virus A, respiratory syncytial virus Type B, parainfluenza virus type 2, Bacillus pertussis, coronavirus OC43, coronavirus HKU1, coronavirus 229E, coronavirus NL63, human rhinovirus did not cross react; Blood was present in the sample and mucin and nasal secretions had no effect on the sample's detection.

High accuracy: Rapid detection of novel coronavirus SARS-CoV-2 and influenza A and B viruses at the same time. Reference test results positive coincidence rate of 100%, negative coincidence rate of 100%.
Simple operation: One-step RT-PCR, complete closed tube amplification and detection, to prevent aerosol pollution. The detection result can be reported in 35 mins.


The kit is used for the auxiliary diagnosis and epidemiological surveillance of SARS-CoV-2, Influenza A Virus and Influenza B Virus infection, cannot be used as the basis for the diagnosis orexclusion of cases alone.


The SARS-CoV-2 and type A/B influenza virus mixed sample with a concentration of 200 copies/mL was detected by the SARS-CoV-2 and type A/B influenza virus nucleic acid detection kit (Fluorescence PCR method) for 20 replicates. The results are shown in the following table and graph:

Result: The results showed that the detection rate of the mixed sample containing 200 copies/mL of SARS-CoV-2, influenza A virus, and influenza B virus was 100% in 20 replicates, indicating that the detection results of the kit at low concentrations were stable and the reproducibility was good.


1. The kit should be stored at -25°C ~ -15°C away from light and avoid repeated freeze-thaw.

2. The kit can be stored for 3 days at 2-8°C after opening.

3. The kit can be stored for up to 12 months if all components are kept in the manner above. Do not use after the stated expiry date.

4. The kit can be transported in foam box sealed with ice bags or dry ice under 8°C.