The kit selects specific conserved regions of AIV H5N1 subtype to design primers and probes. The detection system uses two different fluorophores to independently detect AIV H5 subtype target gene (FAM channel) and AIV N1 subtype target gene (HEX channel).
Sample type: animal plasma, serum, swabs, tissues and virus cultures can be selected.
Ultra-high sensitivity: the detection limit can reach 500copies/mL
High specificity: it can distinguish the subtypes of avian influenza virus, and all tests for influenza B have maintained good negative results.
Wide applicability: it can be used for various fluorescence quantitative PCR instruments on the market, especially for the existing fluorescence quantitative PCR instruments of Bioer Company.
Simple operation and short time-consuming: the kit adopts the form of premix, with few components, which is convenient for operation; the PCR reaction process can be completed in just 1h.
This kit can be used for qualitative testing of avian influenza virus（AIV） H5N1 subtype in throat swabs, cloaca swabs, serum and lung tissue of suspected infection or other animals that require the diagnosis or differential diagnosis of avian influenza virus H5N1 subtype infection (chicken, duck, goose, etc.). The test results of this kit are for clinical reference only and should not be used as the sole basis for diagnosis or exclusion.
Case 1: Use this kit to test positive samples of the H5N1 virus and draw a standard curve.It was found that the correlation coefficient of the CT values detected by each subtype reached more than 0.995, indicating that the linear relationship of the kit is good and the PCR efficiency is high. The results are as follows：
Case 2: Specific detection of avian influenza virus H5N1 subtype nucleic acid detection kit Positive samples of H1N1, H3N2, H5N1, H7N9, H9N2, Type B V, Type B Y, Newcastle disease virus, duck fever virus, chicken infectious Farsi virus, chicken infectious bronchitis virus were tested using a kit. The results showed that the H5N1 subtype of avian influenza could detect H5 subtype and N1 subtype samples, and the rest of the samples were negative; It shows that the kit has no crossover with the above viruses and has good specificity. The results are summarized as follows:
1. The kit need to be transported under freezing conditions.
2. The kit should be stored at -25℃~-15℃ away from light, and avoid repeated freeze-thaw more than 5 times.
3. The kit can be stored for up to 12 months if all components are kept in the manner above. (Please use the kit in the period of validity).
| Cat# | Product Name | Packing size |
| BSL58S1 | Avian Influenza Virus H5N1 Subtype Nucleic Acid Detection Kit (Fluorescent PCR method) | 24T |
| BSL58M1 | Avian Influenza Virus H5N1 Subtype Nucleic Acid Detection Kit (Fluorescent PCR method) | 48T |