Product Details FAQ Specification Instructions

The kit selects specific conserved regions of AIV H5N8 subtype to design primers and probes. The detection system uses two different fluorophores to independently detect AIV H5 subtype target gene (FAM channel) and AIV N8 subtype target gene (HEX channel).

Sample type: available for animal plasma, serum, swab, tissue and virus culture.

High sensitivity: the detection limit can reach 500copies/mL.

High specificity: It can distinguish different subtypes of AIV, and maintain good negative results for the detection of Avian influenza B.

Wide applicability: It can be used in various Real-Time PCR instruments on the market, especially supporting Bioer Real-Time PCR instruments.

Simple operation and short time-consuming: The kit adopts premixed form, with few components, which is convenient for operation; the PCR reaction process can be completed in only 1 hour.

This kit can be used for qualitative testing of avian influenza virus（AIV） H5N8 subtype in throat swabs, cloaca swabs, serum and lung tissue of suspected infection or other animals that require the diagnosis or differential diagnosis of avian influenza virus H5N8 subtype infection (chicken, duck, goose, etc.). The test results of this kit are for clinical reference only and should not be used as the sole basis for diagnosis or exclusion.

Using this kit to detect the H5N8 positive sample and make standard curve, which find the correlation coefficients of the Ct values detected by each subtype are above 0.995, indicating that the kit has a good linear relationship and high PCR efficiency. The results are as follows:

The kit was stored at -20ºC and 37ºC for 7 days, and was taken out to detect positive samples of different concentrations. The results showed that the kits being stored at 37ºC for 7 days, compared to the kits being stored at -20ºC to detected samples of different concentrations at the same time, there was no significant difference in the detection effect, indicating that the stability of the kit was good. The detection result is as follows:

1. The kit need to be transported under freezing conditions.

2. The kit should be stored at -25℃~-15℃ away from light, and avoid repeated freeze-thaw more than 5 times.

3. The kit can be stored for up to 12 months if all components are kept in the manner above. (Please use the kit in the period of validity).