This product is a fast and inhibitor resistant DNA polymerase with a relative molecular weight of 94 kDa. According to enzyme reaction kinetics, it has been specially mutated by genetic engineering. It has a higher elongation rate (elongation rate of 2 seconds /1000bp) and stronger inhibitor resistance. It is especially suitable for rapid amplification of the samples with many inhibitors, which are difficult to be amplified by ordinary Taq enzyme, and can save time and cost. The PCR product amplified by this enzyme has an "A" base attached to its 3 'end, which is suitable for TA cloning. This enzyme has a strong 5'→3' exonuclease activity, which can be used for qualitative PCR and real-time fluorescence quantitative PCR (such as TaqMan™ probe method, SYBR™ method, it is recommended to use the hot start form of this enzyme to improve the detection sensitivity, BSA48S1 of our company is recommended).
Low Host gDNA Residue: Thanks to a unique rTaq purification process, the level of host DNA residue is comparable to that of premium imported brands.
Fast Amplification Speed: 2s/kb.
Excellent Tolerance: Suitable for samples with a high presence of inhibitors, such as humic acid, NaCl, ethanol, isopropanol, and more.
Selecting samples with varying concentrations of humic acid, the comparison shows that BioQuick exhibits significantly better amplification performance than the competitor.
-25~-15℃, protected from light, valid for 12 months.