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Dengue virus belongs to the Flavivirus genus of the Flaviviridae family. It is a single-stranded positive-sense RNA virus with spherical viral particles approximately 55 nm in diameter. Based on antigenic differences, it is classified into four serotypes: 1, 2, 3, and 4.

Dengue virus is primarily transmitted by vector insects such as Aedes aegypti and Aedes albopictus mosquitoes. It causes dengue fever as well as dengue hemorrhagic fever and dengue shock syndrome, which have high morbidity and mortality rates. Typical clinical manifestations of dengue fever include sudden onset of high fever, headache, severe muscle and joint pain (often described as "breakbone fever"), and in some patients, rash, bleeding tendencies, lymphadenopathy, leukopenia, and thrombocytopenia.

Product Details FAQ Specification Instructions

This kit targets highly conserved regions of the dengue virus, designing specific primers and probes that bind specifically to the target sequence. Under the action of reverse transcriptase and hot-start Taq DNA polymerase, this fragment is specifically amplified. During amplification, the fluorescent probe is hydrolyzed, producing fluorescence. The detection system includes dUTP-UDG enzyme contamination prevention measures, thoroughly degrading potential product contamination to avoid false-positive results. Additionally, an internal control is set for a highly conserved region of the human genome, monitoring the entire process of sample collection, transportation, nucleic acid extraction, and PCR amplification. This prevents false-negative results and ensures the effectiveness of the entire process.


Advantages: This kit qualitatively detects dengue virus nucleic acid for clinical diagnosis. It includes contamination prevention to avoid false positives and a human internal control to prevent false negatives. Results are available in 45 minutes.

High Applicability: Suitable for human serum and/or plasma.

High Sensitivity: Detects down to 500 copies/mL, tested with three different reagent batches.

High Specificity: No cross-reactivity with common pathogens like hepatitis B and C, influenza A and B, herpes simplex, encephalitis, and cytomegalovirus.

High Accuracy: Quickly detects dengue virus with 100% accuracy in positive and negative reference samples.


Clinical Diagnosis

Public Health Surveillance

Research Laboratories

Border and Entry Screening

Field Surveys


1. Accuracy: Positive reference samples (P1-P4) and negative reference samples (N1-N8) provided by Hangzhou Bioer Technology Co., Ltd. were reconstituted as required and then extracted using the Bioer extraction reagent BSC86S1E (Zhejiang Medical Device Registration No. 20200872) for subsequent testing.


The results indicate accurate detection of dengue virus nucleic acid. The testing of company reference samples shows a 100% positive agreement rate and a 100% negative agreement rate.

2. Precision: The precision reference samples (J1-J2) of the dengue fever virus nucleic acid detection kit (fluorescent PCR method) were reconstituted according to the instructions for use. After extraction with the Bioer extraction reagent BSC86S1E (Zhejiang Medical Device Registration No. 20200872), testing was conducted using three batches of reagents, with each sample tested 10 times.


The results indicate that the inter-batch and intra-batch precision coefficients of variation for the three batches of reagents are all less than 5%, demonstrating good reagent precision.


-20 ± 5 ℃


Cat# Product Name Packing size
BSJ43M1 Dengue Virus Nucleic Acid Detection Kit (Fluorescent PCR) 48T
BSJ43L1 Dengue Virus Nucleic Acid Detection Kit (Fluorescent PCR) 96T