The Loop-mediated isothermal amplification (LAMP) is a rapid nucleic acid amplification technique. This reagent kit is based on the LAMP principle, using Bst DNA Polymerase and 2-3 pairs of specific primers to generate the target segment through isothermal amplification. During LAMP isothermal amplification, a large number of hydrogen ions are generated, causing the reaction system to change from initially alkaline to acidic. The decrease in pH resulting from successful amplification can be monitored through a color change of a pH-sensitive indicator. This reagent kit contains a pH indicator. It appears purple-red before the reaction, turns orange-yellow in a positive reaction, and remains purple-red in a negative reaction.