This kit designs primer probes in the specific conserved regions of each individual type of foot-and-mouth disease virus, uses polymerase chain reaction (PCR) combined with Taqman fluorescent probe technology to achieve qualitative detection of pathogens, and introduces non-competitive exogenous internal standards for quality control extraction and detection process. The detection system of this kit uses two different labeled fluorophores to independently detect the target gene (FAM channel) and exogenous internal standard gene (HEX channel) of foot-and-mouth disease virus in different wavelength regions, aiming to establish a real-time PCR detection method that can quickly diagnose foot-and-mouth disease virus, so as to achieve the purpose of rapid triage of groups with suspected symptoms, in order to effectively prevent and control the disease of universal infection of foot-and-mouth disease virus.
Ingredients ：
Applicable Samples: Hoofed animal blister fluids, blood, or oral secretions from pigs, cattle, sheep, and other animals.
High Specificity: No cross-reactivity with diseases such as Porcine Blue Ear Virus, Porcine Circovirus, Porcine Pseudorabies Virus, Porcine Parvovirus, Porcine Rotavirus, Porcine Epidemic Diarrhea Virus, Porcine Transmissible Gastroenteritis Virus, and Classical Swine Fever Virus.
High Accuracy: It can accurately detect all seven serotypes of Foot-and-Mouth Disease Virus, producing results that match the expected outcomes.
Simple Operation: The RT-PCR process is completed in a single step, with fully closed-tube amplification and detection, preventing aerosol contamination.
This kit can be used for qualitative testing of foot-and-mouth virus in vesicular fluid, blood, or oral secretions of suspected infection or other animals that require the diagnosis or differential diagnosis of foot-and-mouth virus infection (pigs, cattle, sheep, etc.). The test results of this kit are for clinical reference only and should not be used as the sole basis for diagnosis or exclusion.
The foot-and-mouth disease virus sample was extracted by using Bioer MagaBio plus virus DNA/RNA purification kit III, and the extracted product was stably detected after 20 repeated tests using the kit, and the coefficient of variation of Ct value was less than 1%, indicating that the kit was accurate and reliable for the test results. The test results are as follows:
1. The kit need to be transported under freezing conditions.
2. The kit should be stored at -25℃~-15℃ away from light, and avoid repeated freeze-thaw more than 5 times.
3. The kit can be stored for up to 12 months if all components are kept in the manner above. (Please use the kit in the period of validity).
| Cat# | Description | Packing size |
| BSB47S1 | Foot-and-mouth disease virus universal nucleic acid detection kit (fluorescent PCR method) | 24T |
| BSB47M1 | Foot-and-mouth disease virus universal nucleic acid detection kit (fluorescent PCR method) | 48T |
