The kit selects specific conserved regions of AIV H7N9 subtype to design primers and probes. The detection system uses two different fluorophores to independently detect AIV H7 subtype target gene (FAM channel) and AIV N9 subtype target gene (HEX channel).
Ultra-high Sensitivity: The detection limit can reach 500copies/mL.
High Specificity: lt can distinguish the subtypes of avian influenza virus, and all tests for influenza B have maintained good negative results.
Wide Applicability: lt can be used for various fluorescence quantitative PCR instruments on the market, especially for the existing fluorescence quantitative PCR instruments of Bioer Company.
Simple Operatation and Short Time Consuming: The kit adopts the form of premix, with few components, which is convenient for operation: the PCR reaction process can be completed in just 1h.
This kit can be used for qualitative testing of avian influenza virus（AIV） H5N8 subtype in throat swabs, cloaca swabs, serum and lung tissue of suspected infection or other animals that require the diagnosis or differential diagnosis of avian influenza virus H5N8 subtype infection (chicken, duck, goose, etc.). The test results of this kit are for clinical reference only and should not be used as the sole basis for diagnosis or exclusion.
Positive samples of H1N1, H3N2, H5N1, H7N9, H9N2, Type B V, Type B Y, Newcastle disease virus, duck fever virus, chicken infectious Farsi virus, and chicken infectious bronchitis virus were tested using a kit. The results showed that the H7N9 subtype of avian influenza can detect H7 subtype and N9 subtype samples, and the rest of the samples were negative; it shows that the kit has no cross with the above viruses and has good specificity. The results are summarized as follows:
The positive samples of avian influenza virus H7N9 were repeatedly tested with this kit 20 times. After statistics, it was found that the CT value coefficient of variation (CV) of the test results of each subtype was less than 1%, indicating that the precision of the kit is good, and the test results of the same sample are stable and reliable. The specific results are summarized as follows：
1. The kit need to be transported under freezing conditions.
2. The kit should be stored at -25℃~-15℃ away from light, and avoid repeated freeze-thaw more than 5 times.
3. The kit can be stored for up to 12 months if all components are kept in the manner above. (Please use the kit in the period of validity).
| Cat# | Product Name | Packing size |
| BSL57S1 | Avian Influenza Virus H7N9 Subtype Nucleic Acid Detection Kit (Fluorescence PCR) | 24T |
| BSL57M1 | Avian Influenza Virus H7N9 Subtype Nucleic Acid Detection Kit (Fluorescence PCR) | 48T |