In this kit, RNA viruses synthesize cDNA strands using RNA as templates under the action of reverse transcriptase.Qualitative detection of Avian influenza virus by polymerase chain reaction (PCR) combined with Taqman fluorescence probe technique under the action of polymerase.
Sample Type: Animal plasma, serum, swab, tissue, and virus culture can be selected.
High Sensitivity: The detection limit can reach 200 copies/ml.
High Specificity: It Can effectively detect Avian Influenza virus, and no cross-reaction with Influenza B Virus, Newcastle Disease Virus, Infectious Bursal Disease Virus, Avian Infectious Bronchitis Virus, Avian Infectious Laryngeal Tracheitis Virus, Avian Maorik's Disease Virus, Chicken Mycoplasma, Salmonella, Chicken Genomic DNA.
Wide Applicability: It Can be compatible with various fluorescent quantitative PCR instruments on the market, especially Bioer Real-Time PCR instruments.
Good Stability: The reagent kit adopts the form of lyophillization. After lyophillization, the active substances (such as enzymes) in the product will be protected at ambient temperature, and can be transported at room temperature, improving and extending the stability of the reagent, greatly reducing the problems of reagent performance degradation or failure caused by temperature changes.
Simple Operation and Short Time Consumption: The reagent kit does not need to be prepared, and after re-dissolution, it only needs to be packaged in one step to add samples for testing, and the test results can be obtained within 1 hour.
The Avian Influenza Virus Lyophilized Nucleic Acid Detection Kit (Fluorescence PCR) is utilized in veterinary diagnostics for rapid detection of avian influenza viruses in poultry and wild bird populations, aiding in surveillance and outbreak management.
The standard curve was drawn after using the kit to detect the positive samples of avian influenza virus. The correlation coefficient of Ct value of the target gene of avian influenza virus was 1.000, indicating that the kit had a good linear relationship and high PCR efficiency. The results are shown in the following figure:
1. The unopened kit should be stored at -25℃~8℃ away from light.
2. The kit can be stored for up to 12 months if all components are kept in the manner above. Do not use after the stated expiry date.
3. Don’t open aluminum pouch unless you are ready to use the kit.
4. The lyophilized reagents (reaction mixture) reconstituted with water should be stored at -25℃~-15℃ away from light for up to 1 week. Avoid repeated freeze-thaw (no more than 2 times).
5. The kit can be transported at up to 37℃ for no more than 1 month.
Cat# | Product Name | Packing size |
BSL78S1 |
Avian Influenza Virus Lyophilized Nucleic Acid Detection Kit (Fluorescence PCR) |
48T |