The reagent is designed as a ready-to-use, lyophilizable solution for detection and quantitation of target RNA sequences using hydrolysis probes. This pre-mixed formulation can be flexibly applied to single-target or multiplex RT-qPCR by adding primers, probes and templates. This product contain optimized buffer, Mg2+, dNTPs, hot-start quick Taq DNA polymerase, reverse transcriptase, lyophilized protectants and excipients. Hot-start Taq DNA polymerase and stabilizers result in high amplification efficiency and low non-specific amplification. This product does not contain ROX reference dyes. The reagent is used for lyophilized products which show good lyophilized appearance, reconstitution and stability.
1. Convenient Application: Pre-mixed Master Mix, requiring only the optimization of primers and probes.
2. Good Lyophilization Homogeneity: Can be lyophilized with stability ensured through Sork technology before lyophilization.
3. Short Lyophilization Cycle: Utilizes a three-stage lyophilization process with a cycle of approximately 27 hours.
4. Short Detection Time: RNA sample testing can be completed in under 30 minutes.
Experimental Comparison and Validation of BioEasy 2 × RT qPCR Master Mix (Lyophilizable) and supplier A brand premix were used to test the detection performance of novel coronavirus genes (ORF1ab and N genes). The test samples were the products extracted from novel coronavirus culture, and were diluted with 10 times concentration gradient respectively. The test results are shown in Figure 1, Figure 2 and Table 1.
Results: The results demonstrate that BioEasy 2×RT-qPCR Master Mix (Lyophilizable) as a lyophilized reagent exhibits significantly better detection performance compared to Brand A.
1. The kit should be transported in foam box sealed with ice bags or dry ice at 2-8℃ or lower.
2. Kit storage: Store in a -25~-15°C refrigerator away from light, and the repeated freezing and thawing should not exceed 7 times.
3. Validity period: 12 months, please use within the validity period.
