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Hand-foot-mouth disease (HFMD) is a common infectious disease caused by enterovirus infection, characterized by fever, sore throat, general malaise, rash or herpes on the hands, feet, and mouth. People are generally susceptible to enteroviruses and can be infected in different age groups, mainly in children under 5 years old. Some patients have fatal cardiopulmonary diseases and nervous system complications and may even lead to death.The main enteroviruses that can cause HFMD include Coxsackievirus A (type 2, 4, 5, 6, 7, 9, 10, 16), Coxsackievirus B (type 1, 2, 3, 4, 5), enterovirus 71 and Echovirus. Among them, A16 and enterovirus 71 are the traditional high types, but A6 and A10 have gradually become the main pathogens causing HFMD in recent years.

Product Details FAQ Specification Instructions

This kit uses fluorescent PCR to design primers and probes targeting conserved regions of enterovirus 5 UTR and VP1 genes. The 5 ' of the probe is labeled with the reporting group, and the 3' is labeled with the quenched group. In the processof PCR amplification, when the probe kept the complete sequence, the fluorescence of the group was reported to be absorbed by the quenched group without producing fluorescence; when the probe was combined with the template. the probe would be cut off by Taq enzyme (5 '-3' exonuclease activity); after thegroup was separated from the quenched group, the fluorescence could not be absorbed by the quenched group and produced fluorescence signal. The fluorescence PCR instrument will automatically draw a fluorescence curve according to the detected fluorescence signal and determine the detection result. With human P globin gene as internal reference (IC) gene, this kit can quality control the whole extraction and detection process.


� High sensitivity

The kit can detect Echovirus with detection rate equal or higher than 95% at the concentration equal or higher than 500 copies/mL.

� Accurate diagnosis
The kit is used for qualitative detection of enterovirus universal (EVU, include A2, A4, A5, A6, A7, A9, A10, A12, A16, B1, B2, B3, B4, B5, enterovirus 71 and Echovirus, etc.), and can distinguish enterovirus 71(EV71), Coxsackievirus A6 (CA6), Coxsackievirus A10 (CA10) and Coxsackievirus A16 (CA16).
� Strong specificity
No cross reactivity with Norovirus, Herpes simplex virus 1, Herpes simplex virus 2, Enteric adenovirus, Varicella zoster virus, Rotavirus, Epstein-Barr virus, Rubella virus, Measles virus, Influenza A virus, Parainfluenza virus, Influenza B virus, Human cytomegalovirus, Respiratory syncytial virus, Group B Streptococcus, Klebsiella pneumoniae, Escherichia coli, Streptococcus pneumoniae, Staphylococcus aureus, Salmonella, Haemophilus influenzae, Mycoplasma pneumoniae, Adenovirus and Human genomic DNA , etc.
� Strong stability
Endogenous inhibitors (blood, mucin, etc.) and exogenous inhibitors (contains medications commonly used to treat or treat similar symptoms such as Dexamethasone, Ribavirin , Oseltamivir ,etc.) in the samples had no significant effect on the test results.


This kit can detect coxsackie virus A2, A4, A5, A6,A7, A9,A10, A12,A16. B1.B2. B3, B4, B5, enterovirus 71 and Echovirus, etc. The test results are only used for in vitro diagnostic aid and should not be used asthe sole basis for clinical diagnosis, treatment or management of patients.



1. The kit should be stored at -25°C ~-15°C away from light and avoid repeated freeze-thaw.
2. The kit can be stored for 7 days at 2 ~8 °C after opening.

3. The kit can be stored for up to 12 months if all components are kept in the manner above. Do not use after the stated expiry date.

4. The kit can be transported in foam box sealed with ice bags or dry ice under 8°C.