Influenza A Virus /Influenza B Virus Nucleic Acid Detection Kit (FluorescenceRT-PCR) amplifies and detects viral RNA in swab specimens obtained fromsuspected patients. This product selects the M gene (FAM) region of Influenza AVirus and N gene (HEX) regions of lnfluenza B Virus, and designs two sets ofprimers and fluorescent probes that cover two sites of the genes. The two sets ofprimers and probes can specifically bind to the target sequences. When the RT-PCR amplification reaction is performed, the fluorescent signal(s) can be detected by afull-automatic fluorescent PCR detector to realize real-time online monitoring ofthe RT-PCR reaction. In order to control the entire extraction and detection process,human Ribonuclease gene (CY5) was act as a non-competitive internal controlduring the extraction and detection process.
Strong applicability: suitable for pharyngeal swabs
High sensitivity: up to 200 copies/mL
Strong specificity: no cross-reaction with a variety of common respiratory tract infection pathogens such as meningococci, Haemophilus influenzae, etc. Endogenous inhibitors (blood, mucin, nasal secretions, etc.) and exogenous inhibitors (dexamethasone, budesonide, etc.) in the samples had no significant effect on the test results
High accuracy: The kit can simultaneously detect influenza A virus subtypes:
A H1N1, A H1N1 (2009), A H3N2, A H5N1, A H7N9; influenza B virus: Victoria strain and Yamagata strain. The positive and negative coincidence rates were 100% and 100% respectively.
Respiratory infection disease detection
