In recent years, with the increase of various emerging infectious diseases, it has brought new challenges to the prevention and control of diseases. New technologies are used for rapid laboratory detection of emerging infectious diseases to provide etiological diagnosis basis, in order to achieve rapid and accurate diagnosis and scientific prevention and control.
With the development of molecular biology technology by leaps and bounds, the PCR technology can complete the amplification and analysis of gene or fragment one million times with in several hours and realize the rapid detection of target DNA or RNA, it has the features of fast detection, high sensitivity, strong specificity, simple operation and widely used, etc.
The identification and diagnosis of causesis the most important step for CDC to deal with public health emergencies such as infectious diseases. PCR technology can quickly detect some pathogenic microorganisms that have been isolated and cultured for a long time or can not be isolated and cultured, and provide rapid pathogenic diagnosis results, which is an important basis for the diagnosis of etiology. The application of PCR technology for rapid detection of pathogen samples will be of great significance for disease prevention, especially for rapid and effective treatment of public health emergencies, which will bring significant social and economic benefits and have important scientific research and application value.