This kit is used for in vitro qualitative detection of high-risk human papillomavirus (HPV) nucleic acid DNA in female cervical epithelial cell samples. It is suitable for auxiliary diagnosis and drug treatment efficacy monitoring of 15 high-risk human papillomavirus infections, including 16, 18, 31, 33, 35, 39, 45, 51, 52, 53, 56, 58, 59, 66 and 68, and can simultaneously classify and identify high-risk human papillomavirus type 16 and high-risk human papillomavirus type 18.