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The main symptoms of the novel coronavirus pneumonia are fever and dry cough. Some people will have other symptoms such as fatigue, muscle aches, shortness of breath, diarrhea, etc., but the symptoms such as runny nose and sputum are rare. Influenza A virus is the most common influenza virus. It usually presents with influenza-like symptoms, including fever, sore throat, runny nose, nasal congestion, cough, phlegm, headache, general soreness, fatigue, vomiting and diarrhea in some cases. Influenza B virus often causes localized epidemics, patients will have general malaise, high fever, myalgia and other symptoms, serious patients will have some complications, such as encephalitis, pneumonia and so on.

The clinical symptoms and complications caused by novel coronavirus SARS-CoV-2 and influenza A and influenza B are similar, and they are infectious and harmful. The novel coronavirus SARS-CoV-2 and influenza A and B virus nucleic acid detection kit (fluorescent PCR) can simultaneously detect the infection of SARS-CoV-2, influenza A and influenza B viruses, especially in the stage of viral infection to effectively distinguish virus types, which is conducive to the prevention and treatment of novel coronavirus and influenza A and B viruses. It is of guiding significance for the diagnosis and treatment of novel coronavirus and influenza A/B virus. At the same time, it also has the characteristics of economy and efficiency, and maximizes the allocation of medical resources.

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Experimental data

Precision: The precision reference J1-J2 of the nucleic acid detection kit for SARS-CoV-2 Virus, Respiratory Syncytial Virus, Influenza A Virus and Influenza B Virus (fluorescent PCR method) was redissolved according to the use requirements. After being extracted by the Bioer extraction reagent BSC86S1E, three batches of reagents were used for detection and repeated for 10 times.

Figure 1. Amplification curves of a mixed sample of 200 copies/mL of SARS-CoV-2, Influenza A, and Influenza B viruses.

Novel Coronavirus

Influenza A virus

Influenza B virus

1

36.01

37.88

36.54

2

36.12

36.05

36.24

3

37.00

36.95

36.82

4

35.97

37.29

37.14

5

37.01

37.12

36.39

6

35.65

36.32

36.76

7

36.49

36.01

37.01

8

35.71

36.87

36.72

9

36.20

35.59

36.40

10

35.60

36.43

36.84

11

35.93

36.06

35.74

12

36.00

37.43

36.19

13

36.48

36.88

36.31

14

36.92

36.87

37.01

15

37.31

36.59

36.31

16

36.32

37.48

36.56

17

36.91

35.92

37.71

18

35.93

36.64

36.50

19

36.64

37.23

36.22

20

36.65

36.94

36.26

CV value

1.36%

1.60%

1.15%

Table 1. Amplification data of mixed samples containing 200 copies/mL of SARS-CoV-2 and influenza A/B viruses.

The results showed that the detection rate of the mixed sample containing 200 copies/mL of SARS-CoV-2, influenza A virus, and influenza B virus was 100% in 20 replicates, indicating that the detection results of the kit at low concentrations were stable and the reproducibility was good.


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