Monkeypox virus is a kind of enveloped double stranded DNA virus, which is rectangular in shape and can be cultured and grown in African green monkey kidney cells, causing cytopathic effects. It belongs to the orthopoxviral genus of the poxviridae family. Monkeypox is a zoonotic infectious disease caused by monkeypox virus primers. Its clinical manifestations include high fever, headache, general discomfort, cough, enlarged lymph nodes, accompanied by a rash similar to smallpox throughout the body, and occasional abdominal pain. This kit uses fluorescence PCR method and can be applied to Bioer’s LineGene 9600 Plus (FQD-96A), Bioer’s QuantGene 9600 (FQD-96C) and ABI 7500. It has the characteristics of fast detection speed, good specificity, and high sensitivity.
This kit selects two highly conserved regions of monkeypox virus, the F3L gene (FAM) and the B6R gene (HEX) and designs two pairs of specific primer probes. The primers and probes can specifically bind to the target sequence. Under the action of Taq enzyme, the fragment is specifically amplified, and the fluorescent probe is hydrolyzed during the amplification process to produce fluorescence. The detection system contains dUTP-UDG enzyme anti-pollution measures to fully degrade possible product contamination and avoid false positive results. In addition, internal standards are set up to monitor the entire process of sample collection, transportation, nucleic acid extraction, and PCR amplification, avoiding false negative results, and ensuring the effectiveness of the entire process.