Respiratory tract infection is one of the most common infectious diseases worldwide, most of which are bacterial lower respiratory tract infections. Bacterial infection or secondary infection often causes exacerbation of respiratory disease symptoms. There are many pathogens that can cause respiratory tract infection, among which Streptococcus pneumoniae (SP) and Haemophilus influenzae (HI) are important pathogenic bacteria. Two Bacteria Also Major Bacterial Causes of Community Acquired Pneumonia. This kit adopts the fluorescent PCR method, and is equipped with QuantGene 9600 and LineGene 9600 Plus real-time fluorescent quantitative PCR instruments produced by Hangzhou Bioer Technology Co., Ltd., as well as ABI7500. It has the characteristics of fast detection speed, good specificity and high sensitivity。
This kit selects highly conserved regions of Streptococcus pneumoniae and Haemophilus influenzae, and designs specific primer probes. The primers and probes can specifically bind to the target sequence, and under the action of Taq enzyme, the fragment can be specifically amplified, and the fluorescent Probes are hydrolyzed during amplification, resulting in fluorescence. The detection system contains dUTP-UDG enzyme anti-pollution measures to fully degrade possible product contamination and avoid false positive results; in addition, an internal standard is set, which is a highly conserved region on the human genome, and the internal standard monitors sample collection, transportation, nucleic acid extraction and PCR amplification. Increase the whole process, avoid false negative results, and ensure the effectiveness of the whole process.