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Hand-foot-mouth disease (HFMD) is a common infectious disease caused by enterovirus infection, characterized by fever, sore throat, general malaise, rash or herpes on the hands, feet, and mouth. People are generally susceptible to enteroviruses and can be infected in different age groups, mainly in children under 5 years old. Some patients have fatal cardiopulmonary diseases and nervous system complications and may even lead to death.

The main enteroviruses that can cause HFMD include Coxsackievirus A (type 2, 4, 5, 6, 7, 9, 10, 16), Coxsackievirus B (type 1, 2, 3, 4, 5), enterovirus 71 and Echovirus. Among them, A16 and enterovirus 71 are the traditional high types, but A6 and A10 have gradually become the main pathogens causing HFMD in recent years.

The kit is used for qualitative detection of enterovirus universal (EVU, include A2, A4, A5, A6, A7, A9, A10, A12, A16, B1, B2, B3, B4, B5, enterovirus 71 and Echovirus, etc.) nucleic acid in human throat swab samples, and can distinguish enterovirus 71(EV71), Coxsackievirus A6(CA6), Coxsackievirus A10(CA10) and Coxsackievirus A16(CA16).

Product features

Product parameters
Experimental data

Case 1. The detection kit which was stored at 37℃ for 7 days, and the control group reagents of the same batch, which were normally stored at - 20 ± 5℃, were used to detect different concentrations of norovirus, group A rotavirus and enteric adenovirus samples, and the results are shown in the following chart:

Results: The results showed that Echovirus nucleic acids could be detected accurately. Bioer technology reference test results: positive rate of 100%, negative rate of 100%.

Case 2. 10 times gradient diluted control materials of enterovirus were extracted by MagaBio plus Virus DNA /RNA Purification Kit III（BSC86S1E） and detected by this kit.

Results: The results showed that the correlation coefficient of enterovirus was above 0.995, and the linear relationship was good, indicating that the kit had excellent performance.

Case 3. After purification steps,the precision reference J26J1and J26J2 were detected by this kit. The whole tests were repeated 10 times to verify the precision.

Results: The results showed that the precision variation coefficients of the reagents were all less than 5%, indicating that the reagents had good precision.


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