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Respiratory infection is one of the most common diseases, with adolescents and adults being infected about 2-4 times a year, and children being infected about 6-8 times a year. It is usually accompanied by acute inflammation of the nasal cavity, pharynx, or throat, with most infections being viral, except for a few bacterial infections. SARS-CoV-2 Virus, Respiratory Syncytial Virus, Influenza A Virus and Influenza B Virus have strong transmission power. Epidemiological and clinical symptoms are very similar. Double transmission increases the difficulty of epidemic prevention and control. Therefore, it is an important guarantee for people's health to carry out the detection of COVID-19, influenza virus and other pathogens, do a good job in differential diagnosis, and take targeted prevention and control measures in time. This kit uses fluorescence PCR method to detect the virus. It has the characteristics of fast detection speed, good specificity, and high sensitivity.

This kit uses highly conserved regions of SARS-CoV-2 Virus, Respiratory Syncytial Virus, Influenza A Virus and Influenza B Virus, and designs specific primer probes. The primers and probes can specifically bind to the target sequence. Under the action of reverse transcriptase and hot start Taq DNA polymerase, this segment is specifically amplified, and the fluorescent probe is hydrolyzed during amplification to generate fluorescence. The detection system contains dUTP-UDG enzyme to fully degrade potential product contamination and avoid false positive results; In addition, internal standards are set up to monitor the entire process of sample collection, transportation, nucleic acid extraction, and PCR amplification for highly conserved regions on the human genome, avoiding false negative results and ensuring the effectiveness of the entire process.

Product features

Parameters

Description

Sample Type

Nasopharyngeal swab

Sensitivity

500 copies/mL

Precision

Inter-assay, intra-assay, inter-day and intra-day precision CV less than 5%

Accuracy

Be able to detect SARS-CoV-2, Respiratory Syncytial Virus, Influenza A Virus, Influenza B Virus Nucleic Acid simultaneously

Compatible Instruments

Bioer QuantGene9600 (FQD-96C) and LineGene9600 Plus (FQD-96A)

Detection Time

35 min

Certificate

CE-IVD

Storage Condition

Stored at -20 ± 5 ℃ avoid light

Product parameters
Experimental data
Case 1. Accuracy: after the positive reference products P1-P10 and negative reference products N1-N10 of Hangzhou Bioer Technology Co., Ltd. are reconstituted as required, they are extracted with Bioer extraction

kit BSC86S1E and tested. 

Results: The results showed that the nucleic acid of SARS-CoV-2, Respiratory Syncytial Virus, Influenza A Virus, Influenza B Virus could be accurately detected. the positive coincidence rate is 100%, and the negative coincidence rate is 100%.

Case 2. Precision: The precision reference J1-J2 of the nucleic acid detection kit for SARS-CoV-2 Virus, Respiratory Syncytial Virus, Influenza A Virus and Influenza B Virus (fluorescent PCR method) was redissolved
according to the use requirements. After being extracted by the Bioer extraction reagent BSC86S1E, three batches of reagents were used for detection and repeated for 10 times.

 

Results: The results show that the inter-assay and intra-assay precision variation coefficients of the three batches were all less than 5%, and the reagent precision is great.

Case 3. Linear relationship: Take 10-fold gradient samples of SARS-CoV-2 and use Bioer extraction kit BSC86S1E to extract and detect respectively.


Linear relationship: Take 10-fold gradient samples of Respiratory Syncytial Virus and use Bioer extraction kit BSC86S1E to extract and detect respectively.

Linear relationship: Take 10-fold gradient samples of Influenza A Virus and use Bioer extraction kit BSC86S1E to extract and detect respectively.