Human cytomegalovirus (HCMV) is a member of the Herpesvirus family.The kit using boiling lysis method to distill the CMV, which adopts one pair of primers and a specificity of Human cytomegalovirus-specific probes, using PCR amplification and in vitro internal standard technology to detect Human cytomegalovirus, which could assist the diagnosis and prognosis of Human cytomegalovirus infection.