dBIOZOL is a complete and ready-to-use reagent for the isolation of genomic DNA from samples of animal and plant. The dBIOZOL is based on the a novel chaotropic-detergent lysing solution that hydrolyzes RNA and allows the selective precipitation of DNA from a cell lysate. The advanced DNA isolation method combines both reliability and efficiency with simplicity of the isolation protocol, which is fast and permits isolation of genomic DNA from a large number of samples of small or large volumes. In general, 0.5-4μg genomic DNA per mg tissue can be acquired from up to 80mg tissue with dBIOZOL.
Without additional purification, the isolated DNA can be applied to PCR/Real-time PCR, sequencing, Southern blot, mutant analysis, SNP and other molecular biology and biotechnology applications.
During the isolation, a biological sample is lysed (or homogenized) in dBIOZOL and the genomic DNA is precipitated from the lysate with isopropanol. Following an ethanol wash, DNA is solubilized in elution buffer. The procedure can be completed in 40minutes.
