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Influenza is an acute respiratory infection caused by influenza virus. At present, the influenza viruses that infect humans are mainly influenza A and B viruses. Both are infectious and harmful, and their clinical symptoms are similar after infection.The kit can be used to detect two kinds of influenza virus nucleic acid in one test, and has the features of short time, good specificity and high sensitivity. In addition, the kit add a human internal reference to monitor the entire process of sample collection, transportation, nucleic acid extraction and PCR amplification to ensure the effectiveness of the experiment.The kit can be applied to Bioer’s Fluorescence Quantitative Detection System, LineGene 9600 Plus (FQD-96A) and QuantGene 9600（FQD-96C） . The instrument should contain at least three channels of FAM, HEX (VIC/JOE) and CY5.

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Product features
  • Strong applicability: suitable for pharyngeal swabs.
  • High sensitivity: reagent detection sensitivity up to 200 copies/mL.
  • Strong specificity: no cross-reaction with a variety of common respiratory tract infection pathogens such as meningococci, Haemophilus influenzae, etc. Endogenous inhibitors (blood, mucin, nasal secretions, etc.) and exogenous inhibitors (dexamethasone, budesonide, etc.) in the samples had no significant effect on the test results.
  • High accuracy: The kit can simultaneously detect influenza A virus subtypes: A H1N1, A H1N1 (2009), A H3N2, A H5N1, A H7N9; influenza B virus: Victoria strain and Yamagata strain. The positive and negative coincidence rates were 100% and 100% respectively.
  • Simple operation: one-step method to complete RT-qPCR, fully closed tube amplification and detection to prevent aerosol pollution. The detection can be completed within 35 min.
  • Certificate: CE-IVD
Product parameters
Cat.No Product Name Package
BSJ04S1 Influenza A Virus/Influenza B Virus Nucleic Acid Detection Kit (Fluorescence RT-PCR) 32T
BSJ04M1 Influenza A Virus/Influenza B Virus Nucleic Acid Detection Kit (Fluorescence RT-PCR) 48T
Experimental data

Case 1. Samples with 10-fold gradient of each subtype of influenza A virus and influenza B virus were extracted with MagaBio plus Virus DNA /RNA Purification Kit III and then tested with this kit to verify their performance and linear relationship.



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