This kit is matched with QuantGene 9600 real-time quantitative fluorescence PCR instrument of Bioer Technology, and has the characteristics of rapid detection, good specificity and high sensitivity.The conserved region of SARS-CoV-2 genome(ORF1Ab and N genes) was selected to design 2 specific primers and probes. Different fluorescent groups were labeled on the probe to produce different fluorescence in the reaction system, so as to realize rapid detection.
Fast and accuratet:this kit only takes 35min for one reaction, and one step to complete RT-PCR, closed tube amplification and detection to prevent aerosol pollution.
Strong specificity: no cross reaction with similar respiratory viruses. The presence of blood, mucin and nasal secretions in the sample did not affect the test.
Strong applicability: suitable for human nasopharyngeal swabs and sputum.
High sensitivity: Three different batches of reagents were tested with a sensitivity of up to 200 copies/ml.