For the design of this kit, the wild type sequence tested were selected from the reference genome of SARS-CoV-2(NCBI Reference Sequence: NC_045512.2). After comparison with the Alpha, Beta, Gamma, Delta, Lambd and Omicron variants, the conserved sequences of ORF1ab, N and E genes are used to design specific primers and probes. Combined with LineGene 9600 Plus or QuantGene 9600 real-time fluorescence quantitative PCR instrument of Hangzhou Bioer Technology Co., LTD, it can achieve multiplex-detection, rapid diagnosis, and making sure that the wild-type and all variants are detected.
Strong specificity:No cross reaction with similar respiratory viruses. The presence of blood, mucin and nasal secretions in the sample did not affect the test.
Rapid detection: The kit only takes 40min for a reaction,It is used for rapid triage of people with relevant suspected symptoms.
Multiple-detection: Multiple and rapid detection of ORF1ab, N and E genes of SARS-COV-2 in one test to to validate that the wild-type and all variants are detected.
Strong applicability: Suitable for human nasopharyngeal swabs, oropharyngeal swabs or sputum specimens from suspected cases.
High sensitivity: Three different batches of reagents 5 were tested with a sensitivity of up to 200 copies/ml.
