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This kit is a ready-to-use hot start qPCR probe master mix, the mix containing the optimized PCR buffer and antibody-based hot start Taq enzyme, the activity of Taq DNA polymerase is blocked by antibody before pre-denaturation, and its activity can be rapidly released under high temperature conditions, saving the running time of fluorescent PCR and improving the sensitivity and specificity of amplification. Only primer and template need to be added before reaction, and dUTP/UDG in qPCR mix is specially used for pollution control, which can effectively prevent aerosol pollution of amplification products.
Product features
  • Simple and convenient: Only primer and template need to be added before reaction to reduce the risk of contamination.
  • Anti-contamination: dUTP/UDG in qPCR mix can effectively prevent false positives caused by contamination of PCR products.
  • High specificity:The antibody-based hot start Taq enzyme can effectively inhibit the non-specific amplification of PCR and improve the sensitivity and specificity of the reaction.
  • Wide application:The optimized reaction buffer in qPCR mix can reduce the difference of amplification efficiency caused by different target fragments, and can be amplifies singleplex and multiplex qPCR targets.
Product parameters
Cat.No Product Name Package
BSB70S1 BioEasy Multiplex qPCR Probe Master Mix Ⅰ (UDG plus) 100T
BSB70M1 BioEasy Multiplex qPCR Probe Master Mix Ⅰ (UDG plus) 200T
BSB70L1 BioEasy Multiplex qPCR Probe Master Mix Ⅰ (UDG plus) 500T
Experimental data

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