Adopting nucleic acid amplification technique and combining intermediate fluorescence method, SYBR Green I real-time PCR can do detection and quantification on target gene fast and properly.

The kit offers 2×SYBR Green Mix, including High ROX and other components except for template DNA and primers. During operation, user can do real time PCR reaction directly by adding primers and template DNA, which is very simple. The kit has the advantage of high efficiency in amplification, specificity, good repeatability, and high sensitivity and so on after the improvement of the reaction solution and the application of higher quality enzymes, and it’s high GC sequence reaction and quantify is greatly increased.

Instrument

The reagent can be used in series Real Time PCR detection system of Bioer or the same kinds of the instruments of other companies；Especially suitable for Applied Biosystems 5700, 7000, 7300, 7700, 7900, 7900HT, 7900HT Fast; StepOne™, StepOne Plus™.

• Using of the kit

  • Upside down and mix before using the reagent. Before preparing PCR reagents, please centrifuge the reagent for a few seconds. Please mix, but the speed is less than 1400 rpm.
  • Reaction mixture for real time PCR. (Work in ice).
  • Fully mixing the reaction solution（50ul reaction）, according to 45ul to each 0.2ml PCR reaction tube. Please add 5ul template in each PCR reaction tube. Adding template volume can be adjusted as appropriate but should not be exceeded 10ul.

• Note:

  • Please read this manual carefully before beginning the experiment.
  • For research use only.
  • Keeps all solution containing the fluorescence dye protected from light.
  • Nucleic acid is easy to be contaminated and resulted in to the untrusted result, so the user should strictly do the amplification experiment according to standard PCR process.

• Reagent storage

  This reagent can be stored at -20℃ and need be protected from light in 12 months. The reagent can be stored at 2-8℃ when you use up it in 2 weeks.