The kit provides a very simple, fast and economic way for the isolation of pure high–molecular-weight genomic DNA from tissues, adopting the Genomic DNA Buffer Set. The simple purification procedure, based on the remarkable selectivity of Biospin membrane, allows isolation of high yields of pure genomic DNA less than1.5 hour. It not requires expensive equipment, involves only few steps, and completely avoids the use of toxic and hazardous reagents such as phenol and chloroform. In general, 30 μg genomic DNA can be acquired from up to 50 mg tissue by using this kit.

The pure DNA can be applied extensively in PCR/Real-time PCR, sequencing, Southern blot, mutant analysis, SNP and the others.

• Storage

  • The Protease K is to be stored at 2-8℃, others at 15-25℃.
  • All reagents, when stored properly, are stable for 18 months.

• Apparatus and Materials to Be Supplied by the User

  *  Sterile 2.0ml microcentrifuge tubes                    * 10µl/100µl/1000µl tips

  *  Microcentrifuge capable of 14,000g                   * Absolute ethanol

• Important notes

  • Please add 15.75ml absolute ethanol to PW Buffer and mix thoroughly before the first use.
  • Please add 37.8ml absolute ethanol to wash Binding Buffer and mix thoroughly before the first use.
  • FL Buffer may form precipitates upon storage. In case of precipitate forming, please incubate the buffer at 37℃ until the precipitate has fully dissolved.