BioFast Biozol Reagent is a ready-to-use reagent for the isolation of total RNA from animal or plant tissue. The operation of the kit is very simple and easy. First, add the sample to the grind tube with Biozol for complete lysis. Second, add chloroform to the solution and then centrifuge the grind tube. The homogenate will be separated to three phases: upper aqueous phase, interphase phase and organic phase. RNA remains exclusively in the aqueous phase; the RNA is recovered by precipitation with isopropyl alcohol.

Since the operation of BioFast Biozol is very easy, it can realize the extraction  for several samples simultaneously. And total RNA isolated by BioFast BIOZOL Reagent is free of protein and DNA contamination. It can be used for Northern blot analysis, dot blot hybridization, poly (A)+ selection, in vitro translation, RNase protection assay, RT-PCR assay, cDNA library building and other RNA research.

• Storage and transportation

  • Recommended to store at 2-8 ℃ and the quality guarantee period is up to one year. Recommended to avoid light source so as to achieve optimized effect. Stored at room temperature for a while will not influence the performance of the reagent.
  • The kit can be transported at room temperature.

• Important Notes

  RNase can be introduced accidentally into the RNA preparation at any point in the isolation procedure through improper technique. Because RNase activity is difficult to inhibit, it is essential to prevent its contamination. The following guidelines should be observed when working with RNA.

  • Always wear gloves and respirator in order to prevent RNA degradation.
  • Use sterile, disposable plastic ware and automatic pipettes.
  • All plastic wares and tips should be RNase-free as recommended done as below: After washed, metal ware items can be baked at 150°C for 6－8 hours, while tips or glass and plastic items can be soaked for 2 hours at 37 °C or whole night (12hrs) at room temperature in 0.1%(v/v) diethylpyrocarbonate (DEPC) solution, and autoclaved for 20mins in 151bf/in2 (1.034x105 Pa) autoclave and then baked at 60°C.
  • Build a separate RNA laboratory with the only instruments if possible.