The kit provides a very simple, fast and economic way for the isolation of pure high–molecular-weight genomic DNA from fungus, adopting Genomic DNA Buffer Set. The simple purification procedure, based on the remarkable selectivity of Biospin membrane, allows isolation of high yields of pure genomic DNA in less than 1 hour. It requires no expensive equipment, involves only few steps, and completely avoids the use of toxic and hazardous reagents such as phenol and chloroform. In general, 2.5-30 μg genomic DNA can be acquired from up to 100 mg tissue or up to 5ml yeast culture by using Biospin Fungus Genomic DNA Extraction Kit.

The pure DNA can be applied extensively in PCR/Real-time PCR, sequencing, Southern blot, mutant analysis, SNP and the others.

• Storage

  • The kit should be stored at 15-25℃.
  • All reagents, when stored properly, are stable for 18 months.

• Apparatus and Materials to Be Supplied by the User

  *  Sterile 1.5ml micro centrifuge tubes               * 10µl/100µl/1000µl tips

  *  Microcentrifuge capable of 14,000g              * Absolute ethanol     * Vortex mixer

• Important notes

  1. Please add absolute ethanol to Wash Buffer and mix thoroughly before the first use.
  2. Please add absolute ethanol to E Binding Buffer and mix thoroughly before the first use.
  3. LE Buffer may form precipitates upon storage. If a precipitate has formed, incubate the buffer at 37°C until the precipitate has fully dissolved.
  4. Prepare Sorbitol buffer: 18.217 g Sorbitol and 3.7224 g EDTA-2Na, dissolved in 100 ml of pure water.