The kit is a ready-to-use reagent for the isolation of Virus RNA from different type’s sample. Add RLysis Buffer to the processed sample and adding alcohol will bind RNA to spin column. Then RNA can be easily isolated through several washing and eluting steps.

The kit provides a very simple, fast and economical technique to isolate high quality RNA, and can go high-throughput. The pure RNA can be applied extensively in Northern blot, blotting hybridization, in vitro translation, RNase protect assay, RT-PCR/Real-time RT-PCR analysis, construction cDNA library etc.

• Storage and transportation

  • The kit has demonstrated stability of 24 months when stored at room temperature.
  • The kit can be transported at room temperature.

• Apparatus and materials to be prepared by the user

  *  Sterile 1.5ml microcentrifuge tubes            * 10µl/100µl/1000µl tips

  *  Microcentrifuge capable of 14,000rpm       * 70℃ Water bath      * Vortex mixer

  *  Liquid nitrogen (or ice bath) * Optional: β-Me        * PBS          *Trypsination

  *  Red Blood Cells Lysis Buffer（Cat#BSA06M1）         * Absolute alcohol

• Important note

  • RD Buffer may be precipitated at low temperature, the heated at 37 ℃ for a few minutes, to restore the clarification.
  • DNase Stop Buffer Add the alcohol as the volume marked on bottle label and mix well.
  • Wash Buffer Add the alcohol as the volume marked on bottle label and mix well.

• Technical Information

  Sample	Amount
  Animal tissue	≤30mg
  Culture cells	≤1×108
  White blood cells	≤from 5ml whole blood
  For liquid sample	≤100μl