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The kit is a ready-to-use reagent for the isolation of total RNA from various sources including whole blood, animal/plant tissue, cultured cell and bacteria. Add Solution R2 to the processed sample and transfer the mixture to spin column, and then total RNA can be easily isolated through several washing and eluting steps.The kit provides a very simple, fast and economical technique to isolate high quality RNA, and can go high-throughput. The pure RNA can be applied extensively in Northern blot, blotting hybridization, poly(A)+ selection、in vitro translation、RNase protect assay, RT-PCR/Real time RT-PCR analysis ,construction cDNA library etc.
Product features

  • Storage and transportation

    The kit has demonstrated stability of 24 months when stored at room temperature.The kit can be transported at room temperature.

  • Technical Information

    Method

    Time

    Max volume

    Yield

    Spin column

    7~15min

    750µl

    ≥90％

  • Apparatus and materials to be prepared by the user

    *  Sterile 1.5ml microcentrifuge tubes                      * 10µl/100µl/1000µl tips

    *  Microcentrifuge capable of 14,000rpm                 * Absolute ethanol

    *  Vortex mixer

  • Important note

    Add the ethanol (as the volume marked on bottle label) to the wash buffer and mix them well.

Experimental data


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