bioer PCR molecular

Biospin Gel Extraction Kit provides a simple, rapid and effective method to purify DNA fragments from agarose gel in TAE or TBE buffer. DNA fragments ranged from 60bp to 23kb are purified from (up to 3% standard or high/low-melt) gel using Spin column. The recovery rate is 10~55%(<100bp DNA fragments),90~99%(0.1~10kb DNA fragments),20~70%(﹥ 10kb DNA fragments). Purified DNA can be used directly for kinds of downstream molecular biological experiments such as cloning, sequencing, restriction enzyme digestion and so on.

Product features

Fast and simple; all the work can be finished within 15min.
Safe, non-toxin, no need of phenol/chloroform extraction.
High yield, up to 95％.
Wide recovery range, DNA fragments ranged from 60bp to 23kb can be recovery.

Experimental data

Gel slice containing DNA fragment
1.Sample:1007bp PCR product.1~10# are products after extraction. Marker:DL2, 000 .
Contrast of the absorbance result before and after extraction:
according to the contrast of gray scale, the yield is 95%.

Important Notes ！

Please note that the kinds of gel and gel melting point.
Minimize the size of the gel slice by removing extra agarose,
that can cause increasing the recovery rate. Use fresh electrophoresis buffer on electrophoresis to improve the recovery rate.And please using high-quality agarose to ensure the recovery rate unaffected by agarose quality.

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