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The Listeria monocytogenes PCR Fluorescence Quantitative Detection Kit is an advanced molecular assay designed for the sensitive and specific detection of Listeria monocytogenes DNA using fluorescence-based PCR technology. This kit provides quantitative measurement of Listeria monocytogenes DNA, enabling precise detection and quantification of the pathogen in various samples. Its high sensitivity and specificity make it a valuable tool for food safety testing, clinical diagnostics, and environmental monitoring, where accurate detection of Listeria monocytogenes contamination is crucial for public health. This kit streamlines the detection process with its user-friendly protocol, offering rapid results and contributing to efficient and reliable pathogen detection in diverse applications.

Product Details FAQ Specification Instructions

This reagent kit is used for the qualitative detection of Listeria monocytogenes nucleic acid (DNA) in food samples (meat products, canned goods, etc.). It complies with current domestic detection requirements for Listeria monocytogenes and relevant standards (GB 4789.30-2010). The detection sensitivity of the reagent kit is 10-100 cfu.



  • High Sensitivity: The minimum detection sensitivity of this reagent kit is 10 cfu/ml.
  • Simple Operation, Short Time: Reliable results can be obtained in about 90 minutes, excluding the incubation time.
  • Excellent Specificity: Carefully designed specific primers and probes ensure safe avoidance of cross-reactivity and resulting false positives.
  • Wide Applicability: Compatible with various quantitative PCR instruments available in the market, especially those from Bioer, which already has quantitative PCR instruments.


The Listeria monocytogenes PCR Fluorescence Quantitative Detection Kit is utilized in food safety testing, clinical diagnostics, and environmental monitoring. It enables precise and rapid detection and quantification of Listeria monocytogenes DNA in various samples, including food products, clinical specimens, and environmental samples. This kit plays a crucial role in ensuring the safety of food products, diagnosing Listeria monocytogenes infections in clinical settings, and monitoring environmental contamination, thereby contributing to public health and safety.


1. Perform water sample processing and enrichment according to the Chinese standard GB 8538-2016.
2. Extract nucleic acids from the enriched sample in the enrichment solution.
3. Perform fluorescent PCR amplification.

4. Analyze the results.



1. The kit needs to be transported under freezing conditions.

2. The sample extraction buffer in this kit should be stored at room temperature,the rest of the components should be stored at -25°C- -15°C away from light and avoid repeated freeze-thaw more than 5 times.

3. The kit can be stored for up to 12 months if all components are kept in themanner above. Please use the kit in the period of validity.