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Pertussis is an acute respiratory infection caused by Bordetella pertussis, which was one of the major infectious agents leading to infant mortality worldwide. Adult patients and potential infected persons in the family are the main sources of pertussis infection in children. Compared with immunological methods, PCR detection has higher detection sensitivity and specificity, which is conducive to the early diagnosis of diseases.

This product selects the IS 481 region (FAM) of Bordetella Pertussis, and designs one set of primers and fluorescent probe. The one set of primers and probe can specifically bind to the target sequences. When the PCR amplification reaction is performed, the fluorescent signal can be detected by a full-automatic fluorescent PCR detector to realize real-time online monitoring of the PCR reaction. Greatly improve the detection efficiency and shorten the detection time.

Product features
  • High sensitivity: Three different batches of reagents were used to test and the sensitivity can reach to 2.5 CFU/mL.
  • Highly specific detection: No cross reactivity has been observed by testing the clinical positive specimens such as Influenza A H1N1 virus, Respiratory syncytial virus A/B, Parainfluenza virus, Bordetella Parapertussis, Bordetella bronchiseptica, Influenza A virus, Influenza B virus, Adenoviridae 7, Staphylococcus aureus, TE buffer and Human genomic DNA.
  • CE IVD Certificate Diagnostic Test
  • High Precision: Amplification of the conserved sequences IS481 (specific for both Bordetella pertussis), the positive and negative coincidence rates were 100%.
  • Simple Operation: Detect Bordetella pertussis in a single reaction tube.
  • Strong Applicability: Suitable for human pharyngeal swabs.
Product parameters
Cat.No Product Name Package
BSJ10S1 Bordetella Pertussis Nucleic Acid Detection Kit(Fluorescent PCR) 24T
BSJ10M1 Bordetella Pertussis Nucleic Acid Detection Kit(Fluorescent PCR) 48T
Experimental data

Case 1. The plasmid containing the IS481 gene fragment of Bordetella pertussis were diluted according to a 10-fold gradient and use this kit to detect.


Conclusion: The results showed that the target gene amplification coefficient of Bordetella pertussis plasmid is high and the linear relationship is good, which proves that the product has excellent performance.

Case 2. Bordetella Pertussis bacterial culture was diluted to the minimum concentration and extracted using Bioer MagaBio plus Virus DNA/RNA Purification Kit III, each concentration was tested with 20 replicates by this kit.

Conclusion: The testing data demonstrated that the kit can detect Bordetella Pertussis with detection rate equal or higher than 95% at the concentration equal or higher than 2.5CFU/mL.





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