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This kit is matched with LineGene Mini S (FQD-16B) real-time quantitative fluorescence PCR instrument of Bioer Technology, and has the characteristics of rapid detection, good specificity and high sensitivity. With Bioer Nucleic Acid Release Buffer (BSC102), the samples can be directly amplified without purification steps, which greatly improves the operation convenience and experimental efficiency. The conserved region of SARS-CoV-2 genome was selected to design a specific primer probe. Different fluorescent groups were labeled on the probe to produce different fluorescence in the reaction system, so as to realize rapid detection.

Product features
  • Strong applicability: suitable for human nasopharyngeal swabs and sputum.
  • Fast and accurate: this kit only takes 35min for one reaction, and one step to complete RT-PCR, closed tube amplification and detection to prevent aerosol pollution.
  • High sensitivity: Three different batches of reagents were tested with a sensitivity of up to 200 copies/ml.
  • Strong specificity: no cross reaction with similar respiratory viruses. The presence of blood, mucin and nasal secretions in the sample did not affect the test.
  • No purification process required: with Bioer Nucleic Acid Release Buffer（Cat No.:BSC102), the samples can be directly amplified without purification kit and instrument.
Product parameters
Cat.No Product Name Package
BSJ21S1A/ BSJ21M1A SARS-CoV-2 Nucleic Acid Rapid Detection Kit (Fluorescence RT-PCR) 24T/48T
Experimental data
Case 1. Pseudovirus containing ORF1Ab and N genes of SARS-CoV-2 and pathogen cultures were extracted
with Bioer MagaBio plus Virus DNA/RNA Purification Kit III and diluted according to a 10-fold gradient to

detect.


Result: The results showed that the test of SARS-CoV-2 pseudovirus and the nucleic acid test of pathogen cultures both revealed that there was no interference between FAM, HEX and CY5. 

Case 2. Pathogen cultures of SARS-CoV-2 were extracted with Bioer MagaBio plus Virus DNA/RNA Purification Kit III and diluted according to a 10-fold gradient to detect. 


Result: In the four gradients（every 10 times gradient dilution),  the correlation coefficients of amplification were all above 0.995, 3. and the linear relationship was good.



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