The kit provides a very simple, fast and economic way for the isolating of pure high–molecular-weight genomic DNA from all kinds of bacterial, including Gram-Negative Bacterial and Gram-Positive Bacterial, adopting the Genomic DNA Buffer Set. The simple purification procedure, based on the remarkable selectivity of Biospin membrane, allows isolation of high yields of pure genomic DNA in less than 1 hour. It requires no expensive equipment, involves only few steps, and completely avoids the use of toxic and hazardous reagents such as phenol and chloroform. In general, the kit can get at most 30ug genomic DNA from 5×109 Bacterial cells.The pure DNA can be applied extensively in PCR/Real time PCR, sequencing, Southern blot, mutant analysis, SNP and the others.
Product features
Storage
The Proteinase K is to be stored at 2-8℃,others at 15-25℃.
All reagents, when stored properly, are stable for 18 months.
Apparatus and Materials to Be Supplied by the User
* Microcentrifuge capable of 14,000 × g * Absolute ethanol * Vortex mixer
Important notes
Gram negative bacteria(optional step):Please add158mg Lysozyme to EL Buffer, mix thoroughly for 30seconds or so until the solution is clear, stored at 2-8℃.
Gram positive bacteria: Please add 158mg Lysozyme to EL Buffer, mix thoroughly for 30seconds or so until the solution is clear, stored at 2-8℃.
Please add 10.5ml absolute ethanol to BA Buffer and mix thoroughly before use.
Please add 31.5ml absolute ethanol to Wash Buffer and mix thoroughly before use.
order to optimize the effective result, the appropriate number of bacterial cells is at most 5×109, which OD600 is between 1.0~2.0.
RS Buffer may form precipitates upon storage. If a precipitate has formed, incubate the buffer at 56°C until the precipitate has fully dissolved.
