The kit provides a very simple, fast and economic way for the isolating of pure high– molecular-weight genomic DNA from all kinds of cultured cells, adopting the Genomic DNA Buffer Set. The simple purification procedure, based on the remarkable selectivity of Biospin membrane, allows isolation of high yields of pure genomic DNA in less than 1 hour. It requires no expensive equipment, involves only few steps, and completely avoids the use of toxic and hazardous reagents such as phenol and chloroform. In general, Biospin Cell Genomic DNA Extraction kit is applied for the purification of at least 100 cells.

The pure DNA can be applied extensively in PCR, sequencing, Southern blot, mutant analysis, SNP and the others.

• Storage

  • The PK solution is to be stored at 2-8℃,others at 15-25℃.
  • All reagents, when stored properly, are stable for 18 months.

• Apparatus and Materials to Be Supplied by the User

  *  Sterile 1.5ml microcentrifuge tubes               * 10µl/100µl/1000µl tips

  *  Microcentrifuge capable of 14,000g              * Absolute ethanol     * Vortex mixer

• Important notes

  • Please add absolute ethanol to wash buffer and mix thoroughly before use.
  • In order to optimize the effective result, the appropriate number of cultured cell is between 100 and 5×106, which is suspended in 100μl PBS buffer or 0.9%NaCl.
  • LC Buffers may form precipitates upon storage. If a precipitate has formed, incubate the buffer at 37°C until the precipitate has fully dissolved.