Plasmid isolated with traditional protocol normally contains high level of endotoxins (Lipopolysaccharides or LPS). For transfection of endotoxin sensitive cell lines or microinjection, the endotoxins should be removed before the applications. This kit uses a specially formulated buffer that extracts the endotoxin from the plasmid DNA. Two rounds of extraction will reduce the endotoxin level   to 0.1 EU (Endotoxin) per μg of plasmid DNA. The endofree plasmid midiprep kit provides an efficient endotoxin removal step into the traditional purification procedure to produce transfection grade plasmid DNA.

This kit is designed for fast and efficient purification of plasmid DNA from 3 to 5 mL of E. coli culture. The mini column has a DNA binding capacity of 50 μg. The purified endofree DNA is ready for downstream applications such as transfection of endotoxin-sensitive cell lines, primary cultured cells or microinjection.

• Storage and transportation

  •  Resuspension Buffer should be stored at 2-8°C once RNase A is added. All other materials can be stored at room temperature (22-25°C).
  • The Guaranteed shelf life is 12 months from the date of purchase.

• Apparatus and materials to be prepared by the user

  *  100% ethanol                                                     * High speed centrifuge

  *  1.5/2ml high speed centrifuge tubes             * 10µl/100µl/1000µl tips

• Important note

  • RNase A: Add the RNase A solution to Resuspension Buffer and mix well before use. Store at 2-8°C.
  • Lysis Buffer: Precipitates below room temperature. It is critical to warm up the buffer at 50°C to dissolve the precipitates before use. Keep the cap tightly closed for Lysis Buffer after use.
  • N3 Buffer: Precipitates below 10  ℃,warm up at 37  ℃ to dissolve  the precipitates before use.
  • DNA Wash Buffer: Add the alcohol as the volume marked on bottle label and mix well.