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This kit is used to extract high- purity RNA from FFPE tissue sections, with non-toxic deparaffinization solution, high-performance Lysis  II Buffer to release RNA from FFPE efficiently. The high efficient binding of RNA to our spin matrix while proteins and other impurities are removed by wash buffer. Nucleic acids are easily eluted with sterile RNase free water or RElution Buffer. The purified RNA is ready for downstream applications such as Real-Time PCR,Sec.
Product features

  • Storage and transportation

    • The kit has demonstrated stability of 12 months when the PK Solution should be stored at 2-8℃,DNase I should be stored at -20℃，others at room temperature.
    • The kit can be transported at room temperature, but PK Solution and DNase I need cold chain.

  • Apparatus and materials to be prepared by the user

    *  1.5ml microcentrifuge tubes                        * 10µl/100µl/1000µl tips

    *  Centrifuge capable of ≥14,000g                 * Ethanol（≥95%）

    *  Heating block or water bath

  • Important note

    1）Wash Buffer II: Add the ethanol as the volume marked on bottle label and mix well.

    2）Lysis II Buffer precipitates below room temperature. It is critical to warm up the buffer at 50°C to dissolve the precipitates before use.

    3）PK Solution should be stored at 2°C-8℃.

    4）DNase I should be stored at -20°C.

    5）Carry out all centrifugations at room temperature.

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