The kit provides a very simple, fast and economic way for the isolation of pure high–molecular-weight genomic DNA from fungus, adopting Genomic DNA Buffer Set. The simple purification procedure, based on the remarkable selectivity of Biospin membrane, allows isolation of high yields of pure genomic DNA in less than 1 hour. It requires no expensive equipment, involves only few steps, and completely avoids the use of toxic and hazardous reagents such as phenol and chloroform. In general, 2.5-30 μg genomic DNA can be acquired from up to 100 mg tissue or up to 5ml yeast culture by using Biospin Fungus Genomic DNA Extraction Kit.
The pure DNA can be applied extensively in PCR/Real-time PCR, sequencing, Southern blot, mutant analysis, SNP and the others.