The kit provides a very simple, fast and economic way for the isolation of pure high– molecular-weight genomic DNA from plant tissues, adopting the Genomic DNA Buffer Set. The simple purification procedure, based on the remarkable selectivity of Biospin membrane, allows isolation of high yields of pure genomic DNA less than 1 hour. It not requires expensive equipment, involves only few steps, and completely avoids the use of toxic and hazardous reagents such as phenol and chloroform. In general, 1-30 μg genomic DNA can be acquired from up to 100 mg tissue by using this kit.
The pure DNA can be applied extensively in PCR/Real-time PCR, sequencing, Southern blot, mutant analysis, SNP and the others.