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The kit provides a very simple, fast and effective technique for the isolation of pure plasma DNA. DNA in the sample is released using PK solution and LysisB Buffer. The released DNA is bound exclusively and specifically to the Biospin membrane in presence of LysisB Buffer and ethanol under appropriate salt iron and pH conditions. Denatured protein and other contaminants are removed with  twice washing procedures. The DNA is then eluted from the membrane with the elution Buffer. No any expensive equipments are required, using of toxic or hazardous reagents, such as phenol or chloroform is completely avoided. The pure DNA can be applied extensively in PCR/Real-time PCR, and so on.
Product features

  • Storage and transportation

    • The PK solution must be stored at 2～8℃, other components in the kit may be deposited at room temperature.
    • All components, when stored properly, can keep stable for 18months.
    • The kit can be transported at room temperature.

  • Apparatus and materials to be prepared by the user

    *  1.5ml and 2.0ml sterile micro-centrifuge tubes   * 10µl/100µl/1000µl tips

    *  Micro-centrifuge capable of 12,000×g                * Absolute ethanol (>99%)

    *  Vortex mixer                                                         * Warm bath or metal bath

  • Important note

    Add the ethanol (as the volume marked on bottle label) to WB1 buffer and wash buffer and mix them well.

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