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The kit provides a simple, rapid and effective method for purification of DNA fragments from polyacrylamide gel. The simple purification procedure is based on the remarkable selectivity of Biospin membrane. There need few steps to finish extraction without expensive equipment, as completely avoids using toxic and hazardous reagents such as phenol and chloroform. Purified DNA can be used directly for kinds of downstream molecular biological experiments such as cloning, sequencing, PCR, restriction enzyme digestion, and so on.

Product features

Storage and transportation
- The kit should be stored dry at room temperature（15～25℃）, The kit can be stored for up to 18 months if all components are kept properly.
- The kit can be transported at room temperature.
Apparatus and Materials to Be Supplied by the User

* Sterile 1.5 or 2.0 ml microcentrifuge tubes * 10µl/100µl/1000µl tips

* Absolute ethanol * Microcentrifuge capable of 14,000g * Vortex mixer * Water bath or cooling & heating block

Technical Information

Method	Column volume	DNA size range	Electrophoresis buffer	Incubate temperature	Elution recovery
Spin column	750µl	100bp～10kb	TAE/TBE buffer	65℃	≥99％

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Storage and transportation

Technical Information