The kit is a ready-to-use reagent for the isolation of total RNA from animal tissues, cells, bacteria and others (plant tissues are not recommended). Add RLysis Buffer to the processed sample.RD buffer will remove the protein, then adding alcohol will bind RNA to spin column. The DNA will be destroyed by the DNase I reaction. Then RNA can be easily isolated through several washing and eluting steps.
The kit provides a very simple, fast and economical technique to isolate high quality RNA, and can go high-throughput. The pure RNA can be applied extensively in Northern blot, blotting hybridization, in vitro translation,RNase protect assay, RT-PCR/Real time RT-PCR analysis, construction cDNA library etc.
