Biospin Total RNA Extraction Kit II is suitable for the purification of high-quality total RNA from animal tissues, cultured cells, bacteria, feces, plants, fungi and other samples. The purified RNA can be directly used for Northern blot analysis, dot blot hybridization, in vitro translation, RNase protection analysis, RT-PCR, construction of cDNA library, etc.
| Cat.No | Product Name | Package |
| BSC80S1 | Biospin Total RNA Extraction Kit II | 50T |
| BSC80M1 | Biospin Total RNA Extraction Kit II | 100T |
Case 1
1. Extract 1mL (106/mL) of monolayer cultured cells, 100μL of Elution buffer, use Nanodrop 2000 to measure the concentration and 1% agarose gel electrophoresis to detect the integrity of nucleic acid bands, and compare with similar brands reagents.
|
Reagent brand |
Concentration (ng/μL) |
260/280 |
260/230 |
|
Bioer company |
361.4 |
2.06 |
1.82 |
|
T company |
352 |
2.08 |
1.9 |
|
N company |
356.3 |
2.06 |
1.72 |
Case 2
Extract 50mg of the leaf tissues of Buxus microphylla and Ophiopogon japonicus, 100μL Elution Buffer, use Nanodrop 2000 to measure the concentration and 1% agarose gel electrophoresis to detect the integrity of nucleic acid bands, and compare with similar brands reagents.
|
Sample |
T company |
Bioer company |
||||
|
Concentration (ng/μL) |
260/280 |
260/230 |
Concentration (ng/μL) |
260/280 |
260/230 |
|
|
Buxus microphylla |
204.3 |
2 |
1.03 |
228.9 |
2.12 |
1.6 |
|
Ophiopogon japonicus |
251.8 |
2.13 |
2.15 |
301.5 |
2.12 |
1.26 |
The results show that the extraction efficiency of Bioer's kit is better than that of similar products of other brands.